منابع مشابه
Laboratory-directed protein evolution.
Systematic approaches to directed evolution of proteins have been documented since the 1970s. The ability to recruit new protein functions arises from the considerable substrate ambiguity of many proteins. The substrate ambiguity of a protein can be interpreted as the evolutionary potential that allows a protein to acquire new specificities through mutation or to regain function via mutations t...
متن کاملPERSPECTIVE Directed Evolution of Novel Protein Functions
Directed evolution has been successfully used to engineer proteins for basic and applied biological research. However, engineering of novel protein functions by directed evolution remains an overwhelming challenge. This challenge may come from the fact that multiple simultaneously or synergistic mutations are required for the creation of a novel protein function. Here we review the key developm...
متن کاملRESEARCH PROPOSAL: BALAMURALI M M Protein Engineering: Construction of Recombinant Protein Library by Laboratory Based Directed Evolution Approach
Proteins are the building blocks of life. Proteins have to be properly folded in order for them to exhibit their function. There are several diseases associated with protein misfolding and aggregation. The most commonly observed one is the alziemer’s disease. It is still a wonder how proteins fold so fast ranging from microseconds to milliseconds and seconds. Intensive research has been carried...
متن کاملDesign of protein function leaps by directed domain interface evolution.
Most natural proteins performing sophisticated tasks contain multiple domains where an active site is located at the domain interface. Comparative structural analyses suggest that major leaps in protein function occur through gene recombination events that connect two or more protein domains to generate a new active site, frequently occurring at the newly created domain interface. However, such...
متن کاملOptimizing non-natural protein function with directed evolution.
Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit from further optimization. Evolutionary methods can complement these approaches: recent work combining unnatural amino acid mutagenesis and phag...
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ژورنال
عنوان ژورنال: Microbiology and Molecular Biology Reviews
سال: 2005
ISSN: 1092-2172,1098-5557
DOI: 10.1128/mmbr.69.3.373-392.2005